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Load digested DNA samples in 1% agarose gel 3. The fragments produced have a known sequence that can be ligated to complementary sequences and be cloned by the cell’s molecular machinery.
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  • Herein, we report an easy and efficient ligation and restriction enzyme independent (LREI) cloning method for cloning influenza gene segments into pHW2000 vector.
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    Plasmid DNA minipreps are fundamental techniques in molecular biology.

  • Plasmids are frequently used for gene cloning purposes.
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    The highest band size was at 500 base pairs (bp), and the lowest band size was at 75 bp.

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